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Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis


Dent, Maja; Dragović-Uzelac, Verica; Penić, Marija; Jukić, Marijana; Halambek, Jasna; Levaj, Branka
Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis // 15th World Congress of Food Science and Technology (IUFOST 2010 ) : Proceedings / Robertson, Gordon ; Lupien, John (ur.).
Cape Town, Južoaafrička republika: IUFOST, 2010. (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Determination of total phenols and flavonoids from sage (Salvia officinalis L.) using acid hydrolysis

Autori
Dent, Maja ; Dragović-Uzelac, Verica ; Penić, Marija ; Jukić, Marijana ; Halambek, Jasna ; Levaj, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th World Congress of Food Science and Technology (IUFOST 2010 ) : Proceedings / Robertson, Gordon ; Lupien, John - : IUFOST, 2010

ISBN
978-0-9810247-0-7

Skup
IUFoST 2010 15th World Congress of Food Science and Technology

Mjesto i datum
Cape Town, Južoaafrička republika, 22-26.08.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Sage; phenolic acid; flavonoids; acid hydrolysis

Sažetak
Phenolic compounds were investigated in wild sage (Salvia officinalis L.). Conventional extraction (CE) in water bath shaking, extraction in an ultrasonic water bath (US) 35 kHz at 60 °C, 30 minutes and microwave assisted extraction (MAE) power 500 W at 60 °C, 10 minutes were compared. Extractions were performed using 30% ethanol and acetone with the use of acid hydrolysis with 10% and 20% HCl. Sage was harvested on the Mediterranean part of Croatia, dried in the shade and stored in paper bags. Total phenols (TP) were determined spectrophotometrically with Folin-Ciocalteu method. The flavonoids (FL) assay is based on the spectrophotometric evaluation of a complex with AlCl3. The influence of solvents on the amount of extracted TP and FL, and the application of acid hydrolysis with 10% and 20% HCl depending on the applied extraction were examined. Comparing the solvents used for extraction, 30% ethanol extracted higher amount of TP and FL than 30% acetone. Combined 30% ethanol as solvent with acid hydrolysis (10 % HCl) and MAE obtained the highest amount of TP (90.07 mg GAE / g dm), followed by US extraction (78.22 mg GAE/g dm), while CE produced lower amount of TP (62.78 mg GAE/g dm). Conclusions: Using MAE with acid hydrolysis the amount of TP and FL increased and was higher then the one obtained with CE. Application of acid hydrolysis with US extraction decreased the amounts of TP and FL. Extracts obtained with 30% ethanol and acetone without addition of HCl obtained approximately equal value of TP and FL. Acid hydrolysis with 10% HCl increased TP and FL, while the amounts decreased with application of 20% HCl .

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Prehrambena tehnologija



POVEZANOST RADA


Projekt / tema
058-0000000-3488 - Biološki aktivni spojevi u nekim vrstama samoniklog i kultiviranog bilja (Verica Dragović-Uzelac, )
058-0582261-2253 - Ekološke priprave i elektrokemijska karakterizacija biološki aktivnih spojeva (Marijana Jukić, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb