engleski

Genetic diversity of European genotype PRRSV in Central and Eastern Europe - An update

In the present study were determined the ORF7 sequences of PRRSV from recently obtained samples from Latvia, Lithuania, Belarus, Ukraine, Russia, Poland, Hungary, Croatia and Serbia. From samples of serum, aborted fetuses or lungs total RNA was extracted, complete ORF7 sequences were amplified and sequenced with different methods. A total of 28 complete ORF7 sequences representing field PRRSV strains from, Latvia (1), Lithuania (3), Belarus (3), Ukraine (1), Poland (5), Hungary (8), Croatia (4) and Serbia (3) were obtained and all of them were classified to the European genotype. Their analysis showed that Polish, Hungarian, Croatian and Serbian strains were closely related for subtype 1 strains. This collaborative report provides another evidence for already well documented extreme diversity of EU-PRRSV. In summery, the presented data confirm that the strains from Central and Western Europe are closely related and have common origin while the strains from East Europe are much more diverse.

PRRSV; PCR; genotyping

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