engleski

Investigation of reproductive failures caused by porcine circovirus type 2 in large pig production units in Croatia

The objective of this study was to investigate role of PCV2 in aborted and premature piglets connected with reproductive disordes on large pig production units in Croatia. A total of 83 fetuses and premature piglets were included in this study. During postmortem examination samples of heart were collected for histopatological, immuhistochemistry (IHC)and in situ hybridization (ISH)examination. For PCR detection samples of lungs, spleen and liver were collected. Histopathologically, in 21 (25, 3%) fetuses mild miocardial degeneration and mild myocardial necrosis without lymphocytic inflammatory reaction were observed. All of tested samples of hearth were negative for presence of PCV2 antigen by IHC and all of tested myocardium tissue was negative for presence of PCV2 genome by ISH. In all 83 tested samples PCV2 genomic DNA is detected. According to absence of specific gross lesions on examined fetuses, no characteristic histopathological lesions and negative results of IHC and ISH in myocardial tissue we consider there is no evidence that PCV2 caused reproductive failure in Croatian pig production units.

PCV2; reproductive failure

nije evidentirano