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Pregled bibliografske jedinice broj: 481435

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects


Serrano, José; Čipak, Ana; Boada, Jordi; Gonzalo, Hugo; Cacabelos, Daniel; Cassanye, Anna; Pamplona, Reinald; Žarković, Neven; Portero-Otin, Manuel
Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects // Acta biochimica Polonica, 57 (2010), 2; 193-198 (međunarodna recenzija, članak, znanstveni)


Naslov
Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

Autori
Serrano, José ; Čipak, Ana ; Boada, Jordi ; Gonzalo, Hugo ; Cacabelos, Daniel ; Cassanye, Anna ; Pamplona, Reinald ; Žarković, Neven ; Portero-Otin, Manuel

Izvornik
Acta biochimica Polonica (0001-527X) 57 (2010), 2; 193-198

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gallic acid; antioxidant; pro-oxidant; peroxidase; HMEC-1

Sažetak
A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )
108-0000000-0028 - Oksidacijski stres i tumori središnjeg živčanog sustava (Kamelija Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE