Napredna pretraga

Pregled bibliografske jedinice broj: 481426

Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen


Čipak, Ana; Mrakovčić, Lidija; Ciz, Milan; Lojek, Antonin; Mihaylova, Boryana; Goshev, Ivan; Jaganjac, Morana; Cindrić, Marina; Sitić, Sanda; Margaritoni, Marko et al.
Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen // Acta biochimica Polonica, 57 (2010), 2; 165-171 (međunarodna recenzija, članak, znanstveni)


Naslov
Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen

Autori
Čipak, Ana ; Mrakovčić, Lidija ; Ciz, Milan ; Lojek, Antonin ; Mihaylova, Boryana ; Goshev, Ivan ; Jaganjac, Morana ; Cindrić, Marina ; Sitić, Sanda ; Margaritoni, Marko ; Waeg, Georg ; Balić, Marija ; Žarković, Neven

Izvornik
Acta biochimica Polonica (0001-527X) 57 (2010), 2; 165-171

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SUM159; breast cancer stem cells; 4-hydroxynonenal; extracellular matrix; collagen; oxidative homeostasis

Sažetak
Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44+CD24–/lowESA+ usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44+CD24–/lowESA+ cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 μM) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as “second messenger of free radicals”, which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )
108-0000000-0028 - Oksidacijski stres i tumori središnjeg živčanog sustava (Kamelija Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE