engleski

Serological, Bacteriological and Molecular Diagnosis of Brucellosis in Domestic Animals in Croatia

Aim To present the surveillance data on Brucella melitensis, B. suis, and B. ovis infection in cattle, sheep, goats, and swine in Croatia obtained in 2008 by serological, bacteriological and molecular methods for diagnostics of brucellosis in domestic animals. Methods We serologically tested 42785 cattle serums, 22686 sheep and goat serums and 28520 swine serums using the Rose Bengal test, complement fixation test and various immunosorbent assays. We also tested 10173 ram blood samples for B. ovis infection using the complement fixation test. Bacteriological examination was conducted on 214 samples collected from 34 serologically positive animals. Different molecular methods were employed in the identification and typing of 20 isolates from the samples Results B. melitensis biovar (bv.) 3 was confirmed with different identification methods in 2 flocks in 2 Croatian counties. B. melitensis in cows was confirmed for the first time in Croatia. Infection with B. ovis was serologically confirmed in 202 rams in 12 counties. Conclusions In 2008, the size of the brucellosis-affected area in Croatia and the efficiency of detection and prevention of brucellosis in sheep, goat and swine was satisfactory. Infection with B. melitensis in cattle was confirmed for the first time in Croatia and possible links for infection in humans detected. More efficient measures for suppression and control of ovine epididymitis are required and a new strategy may be necessary for complete eradication of this disease.

serology; bacteriology; molecular diagnosis; brucellosis; domestic animals; zoonoses; Croatia

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