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Pregled bibliografske jedinice broj: 478825

Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood


Šimat, Vida; Dalgaard, Paw
Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood // LWT - Food Science and Technology, 44 (2011), 2; 399-406 doi:10.1016/j.lwt.2010.08.011 (međunarodna recenzija, članak, znanstveni)


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Naslov
Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood

Autori
Šimat, Vida ; Dalgaard, Paw

Izvornik
LWT - Food Science and Technology (0023-6438) 44 (2011), 2; 399-406

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
histamine; biogenic amines; HPLC columns with small particle-size

Sažetak
Pre-column and post-column HPLC derivatization methods were modified and evaluated for the identification and quantification of nine biogenic amines in seafood. Two HPLC methods with column particles of 1.8 µm or 3 µm in diameter were modified and compared to classical methods using 5 µm column particles. Both pre-column derivatization with dansyl chloride and post-column derivatization with O-phthalaldehyde were studied. The HPLC methods were compared with respect to the time of elution, eluent consumption, back-pressure as well as separation, sensitivity, recovery and repeatability for determination of biogenic amines in lean canned tuna and fatty frozen herring. The modified methods using smaller column particles of 1.8 µm or 3 µm allowed biogenic amines to be separated and quantified faster (23-59%) and with less eluent consumption (59-62%) than classical HPLC methods. Back-pressures were below 170 bar and this allowed the use of classical HPLC systems rather than dedicated and costly ultra-high-pressure liquid chromatography (UHPLC) equipment. Biogenic amine separation, sensitivity, recovery and repeatability for the modified methods were similar to or performed better than for the classical HPLC methods. Due to reduced analyses time and eluent consumption the HPLC methods would improve biogenic amine analysis.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Ustanove:
Sveučilište u Splitu

Profili:

Avatar Url Vida Šimat (autor)

Citiraj ovu publikaciju

Šimat, Vida; Dalgaard, Paw
Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood // LWT - Food Science and Technology, 44 (2011), 2; 399-406 doi:10.1016/j.lwt.2010.08.011 (međunarodna recenzija, članak, znanstveni)
Šimat, V. & Dalgaard, P. (2011) Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood. LWT - Food Science and Technology, 44 (2), 399-406 doi:10.1016/j.lwt.2010.08.011.
@article{article, year = {2011}, pages = {399-406}, DOI = {10.1016/j.lwt.2010.08.011}, keywords = {histamine, biogenic amines, HPLC columns with small particle-size}, journal = {LWT - Food Science and Technology}, doi = {10.1016/j.lwt.2010.08.011}, volume = {44}, number = {2}, issn = {0023-6438}, title = {Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood}, keyword = {histamine, biogenic amines, HPLC columns with small particle-size} }
@article{article, year = {2011}, pages = {399-406}, DOI = {10.1016/j.lwt.2010.08.011}, keywords = {histamine, biogenic amines, HPLC columns with small particle-size}, journal = {LWT - Food Science and Technology}, doi = {10.1016/j.lwt.2010.08.011}, volume = {44}, number = {2}, issn = {0023-6438}, title = {Use of small diameter column particles to enhance HPLC determination of histamine and other biogenic amines in seafood}, keyword = {histamine, biogenic amines, HPLC columns with small particle-size} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • BIOSIS Previews (Biological Abstracts)
  • CA Search (Chemical Abstracts)
  • FSTA: Food Science and Technology Abstracts
  • Cambridge Scientific Abstracts
  • IFIS
  • Dairy Science Abstracts


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