engleski

Culture conditions and genome instability in callus of Vicia faba

The dependence of genome instability on various nutrient media as well as on the condition of subculturing was studied in three strains of Vicia faba callus culture. Growth rate of maintained callus cultures (strains VFS1, VFZ6 and VFT2) were high on the 3 following media: 1. RM-1962, 3% sucrose, 0.2 mgl-1 2, 4-D and 0.2 mgl-1 kinetin ; 2. RM-1962, 3% sucrose, 0.3 mgl-1 2, 4-D ; 3. B5 modified medium (mineral salts double amount without NH4NO3), 3% sucrose, 0.5 mgl-1 2, 4-D and 5.0 mgl-1 kinetin. Karyological analyses of the three strains showed that in young as well in in old subcultures there were diversity of ploidy levels and/or aneuploidy. The beginning subcultures of the strains VFS1 and VFZ6 (obtained from immmature embryos of unripe seeds) showed, however, a larger variability of polyploidy than the beginning subcultures from the strain VFT2 (obtained from mature embryo of ripe seeds). The increase of kinetin concentrations reduced the frequency of polyploids in favour of diploids (hypo- and hyperdiploids respectively). In addition to the instability of chromosome numbers structural changes in chromosomes, especially translocations and inverzions have been observed by the Giemsa C-banding technique. The predominant chromosome numbers were 14-18 with 3Mchromosomes.

callus culture; Vicia faba; genome instability

nije evidentirano