engleski

Genotyping analysis of 1300-year-old human remains found in Naklice (South Croatia) burial site

Human population genetics has been used in the exploration of the human past together with other techniques to better define genetic, linguistic and cultural inheritance of various populations. Excavation of 19 ancient graves at Naklice (Southern Croatia) has provided unique opportunity to investigate the gene pool ethnogenesis of ancient Southern Croatia population. The aim of this study was to determine the hypervariable region 1 (HV1) sequence of the ancient DNA extracted from the Naklice burial site skeletons and to assign them to appropriate haplogroups. Ancient DNA was extracted from skeletons’ teeth/bones found in a graveyard. All samples were analyzed to determine the autosomal short tandem repeats (STR) profile of each skeleton. This was accomplished by sequencing the hypervariable region 1 (HV1) of the mitochondrial control DNA. Mitochondrial HV1 DNA region was successfully amplified from 27 out of 35 skeletal remains. Each of the 27 mtDNAs was compared to the Cambridge Reference Sequence and was assigned to an appropriate haplogroup. Haplogroup assignment revealed that 18 haplotypes belonged to haplogroup H (67 %), 5 (18%) belonged to haplogroup J, 3 (11%) belonged to haplogroup U5 and 1 (4 %) belonged to haplogroup HV. The frequency of haplogroups differs from the frequency of the same haplogroups in living population of Croatia. This study provides the first ancient DNA sequence analysis from Southern Croatia and could be used as basis for further studies of ancient gene pool of Southern Croatia populations.

Southern Croatian early medieval population; ancient DNA; mtDNA; HV1; STR

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