engleski

Comparison of Diagnostic Value of Indirect Immunofluorescence Assay and Desmoglein ELISA in the Diagnosis of Pemphigus

Pemphigus vulgaris (PV) and pemphigus foliaceus (PF) are autoimmune blistering diseases characterized by intraepidermal separation which is result of autoantibodies directed to desmoglein 1 and desmoglein 3, adhesion molecules that have pathogenic role in blister formation. Both, PV and PF are diagnosed according clinical picture, histopathologic, immunopathologic and molecular biologic features. In our study we compared values of indirect immunofluorescent (IIF) test and enzyme linked immunosorbent assay (ELISA) for desmoglein 1 (Dsg 1) and desmoglein 3 (Dsg 3) at baseline visit only. This study was performed as retrospective study in which 22 patients were included, 19 of which having PV and 3 of them with PF. Sera of all patients were tested with IIF and ELISA Dsg 1 and Dsg 3. In the group of 19 patients with PV, 12 patients had positive IIF, Dsg 3 and Dsg 1 ELISA tests ; 2 of them had positive IIF and positive anti Dsg 3 but negative anti Dsg 1 ; 3 of them had negative IIF but positive both Dsg 1 and Dsg 3 antibodies, and 2 of them had negative IIF and Dsg 1, but positive Dsg 3 antibodies. In the group of patients with PF all three patients had positive indirect immunofluorescence test, positive Dsg 1 ELISA test and negative Dsg 3 ELISA test. Results of our study supported previous studies which confirmed that Dsg 1 and Dsg 3 ELISA is sensitive and specific tool for diagnosis of PV and PF.

pemphigus vulgaris; pemphigus foliacues; indirect immunofluorescence test; anti desmoglein ELISA test

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