Korelacija aktivnosti katalaze i sinteze aflatoksina Aspergillus flavusa (CROSBI ID 359434)
Ocjenski rad | diplomski rad
Podaci o odgovornosti
Zlatar, Dragana
Klapec, Tomislav
Šarkanj, Bojan
hrvatski
Korelacija aktivnosti katalaze i sinteze aflatoksina Aspergillus flavusa
Cilj ovog rada bio je utvrditi da li postoji korelacija aktivnosti katalaze Aspergillus flavusa uzgojenog na podlogama koje potiču ili suprimiraju sintezu aflatoksina. Istraživanje je provedeno na podlozi GMS koja sadrži glukozu i podlozi PMS s peptonom u podlozi. Koncentracija aflatoksina je nakon uzgoja plijesni, ekstrakcije i prečišćavanja određena HPLC-om. Na osnovi rezultata istraživanja utvrđen je viši stupanj konverzije glukoze u biomasu plijesni u odnosu na pepton. Kod PMS podloge utvrđeno je ukupno najviše 23 ppb aflatoksina, dok je na GMS podlozi koncentracija dosegla i 333 ppb. Pepton u hranjivoj podlozi predstavlja izvor dušika, pa je PMS podloga pogodnija je za sintezu proteina što bi moglo objasniti nižu aktivnost katalaze na GMS podlozi. Aktivnost katalaze i količina aflatoksina ponašaju se obrnuto proporcionalno. Na GMS podlozi koeficijent korelacije iznosi -0, 91, sličan koeficijentu korelacije na PMS podlozi koji iznosi -0, 94. Veliki pad aktivnosti katalaze je možda uzrokovao oksidativni stres u stanici plijesni koja na to odgovara pojačanom sintezom aflatoksina.
katalaza; aflatoksini; Aspergillus flavus; oksidativni stres; HPLC
nije evidentirano
engleski
Correlation between catalase activity and aflatoxin synthesis in Aspergillus flavus
The aim of this study was to determine whether there is a correlation between catalase activity of Aspergillus flavus, grown on substrates which either promote or inhibit the synthesis of aflatoxins. The GMS medium was used which contains glucose as well as the PMS medium with peptone. The concentration of aflatoxins was determined by HPLC following cultivation of mold, extraction, and purification. Based on the results, glucose has a higher degree of conversion to mold biomass than peptone. A total of 23 ppb aflatoxin was detected in the PMS medium, while the GMS had a significantly higher amount of up to 333 ppb. Pepton is a source of nitrogen in beef broth and which made the PMS medium more suitable for protein synthesis. Consequently, catalase activity in the GMS medium is less compared to the PMS. Catalase activity and the amount of aflatoxins were inversely proportional. Correlation coefficient in the GMS medium was -0.91, similar to the correlation coefficient in the PMS medium (-0.94). Major decline of catalase activity may have brought about cellular oxidative stress prompting molds to aflatoxin synthesis.
catalase; aflatoxins; Aspergillus flavus; oxidative stress; HPLC
nije evidentirano
Podaci o izdanju
48
04.05.2010.
obranjeno
109:545926
Podaci o ustanovi koja je dodijelila akademski stupanj
Prehrambeno-tehnološki fakultet Osijek
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