engleski

Differential gene expression in visceral and subcutaneous tissue of obese women

Obesity is multifactorial, polygenic, heterogeneous disorder with a complex etiology. Abdominal or visceral obesity is associated with a higher risk for development of obesity related complications, when compared to peripheral, subcutaneous obesity. The aim of the present study was to evaluate differentially expressed genes in visceral and subcutaneous tissue of obese women. The samples of visceral and subcutaneous adipose tissue from 5 Caucasian obese women were collected during cholecystectomy. In total, 215 genes exhibited differential expression between these two fat depots. Of those, 103 had higher expression levels in subcutaneous fat, whereas 112 genes were expressed at higher levels in visceral fat. Differentially expressed genes included those involved in cytokine/receptors, cell adhesion, cell cycle, transcription, signal transduction, transport, cell growth and morphogenesis. The members of HOX gene network exhibited higher expression in subcutaneous adipose tissue. The Wnt signaling pathway related-genes, WNT5A and FZD7, were expressed at higher levels in visceral fat compartment. Enzymes involved in lipid metabolism, such as FADS3, MGLL, LIPE and ACOT4 were highly expressed in subcutaneous fat depot. These results show significant differences in gene expression between visceral and subcutaneous tissue of obese women, indicating putative mechanisms in the development of obesity related disorders.

subcutaneous adipose tissue; obesity; gene expression

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