engleski

Effect of pentadecapeptide BPC 157 on myocardial infarction in rat induced by isoprenaline hydrochloride

We focused on myocardial infarction in rat induced by isoprenaline and possible protective effect of gastric pentadecapeptide BPC 157. Isoprenaline myocardial infarction suitably mimicks human myocardial ischemia. As an anti-ulcer peptide that also improves wound healing (including transected muscle), BPC 157 was shown as safe in trials for inflammatory bowel disease (PL14736, Pliva). This peptide also showed positive effect in heart failure model induced by doxorubicine and heart arryhthmias induced by hypoxic and reoxygenation injury. It can be used without a carrier needed for other peptides, both locally and systemically. Wistar rats received for two consecutive days: (1) saline solution only and/or pentadecapeptide BPC 157 (10μg/kg/day) (ip), (2) isoprenaline only (150mg/kg/day sc) (ISO) (control) and (3) isoprenaline (150mg/kg/day sc) and pentadecapeptide BPC 157 (10μg/kg/day ip) (ISO+BPC 157). BPC 157 was administrated 30min before ISO. Assessment included clinical, microscopy, ECG and biochemistry (i.e., cardiac troponin T (cTnT)) status at 24 or 48 hours. In generally, BPC 157 reduced the isoprenaline induced disturbances. For illustration, after 48 hours, the cTnT values were significantly increased in ISO group compared with healthy [1.4±0.2 ng/mL versus 0.46±0.1 ng/mL, p<0.05]. In BPC 157+ISO group the mean cTnT values were significantly reduced compared with ISO group [0.75±0.08 ng/mL versus 1.4±0.2ng/mL, p<0.05]. ECG changes (ST elevation/depression) were less expressed in ISO+ BPC 157 [ 0.3±0.1mV] compared with ISO group [ ST elevation/depression 1.2±0.1mV] [ p<0.05] . The cTnT values positively corelatte with histological distribution of myocardial cell demage. Thus, ISO+BPC 157 group showed less hystological changes, compared with ISO group. This study has demonstrated protective effect of pentadecapeptide BPC 157 on myocardial infarction induced by isoprenaline in rats and its potentional clinical application in treatment of ishemic heart disease. The results of this study are in accord with previously demonstrated protective effect on endothelial cells and induction of NO by this peptide in various organ's leasons.

BPC 157; myocardial infarction; isoprenaline hydrochloride; rats

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