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Common aberrations from the normal human plasma N-glycan profile

After performing hydrophilic interaction and weak anion exchange HPLC to analyze N-glycans in plasma of 1991 people we identified several individuals that differed significantly from the “normal” profile of N-glycans. By performing consensus scoring of pair wise distances between vectors containing measured glycan values we formed six groups of individuals with specific glyco-phenotypes. Some aberrations from the normal plasma protein patterns were found to be associated with clinical conditions (such as renal problems in people with increased monosialylated biantennary glycans, A2G2S1), while other substantial changes in N-glycan structure, such as the near complete absence of neutral glycans or antennary fucosylated tri- and tetraantennary glycans, were not associated with any observed adverse health outcomes. These results demonstrate the existence of specific altered glyco-phenotypes in some individuals, and indicate that in some cases they might represent risk factors for the development of specific diseases.

protein glycosylation / N-glycans / human plasma glycome / glycan analysis

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