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Diversity of Y-STRs in the Representative Samples of the Local Human Population of Canton Sarajevo


Cenanovic M.; Kovačević, Lejla; Pojskić, Naris; Avdic J.; Marjanović, Damir
Diversity of Y-STRs in the Representative Samples of the Local Human Population of Canton Sarajevo // European Journal of Human Genetics Vol 17: Supplement 2
Beč, Austrija: Nature Publishing Group, 2009. str. 281-281 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
Diversity of Y-STRs in the Representative Samples of the Local Human Population of Canton Sarajevo

Autori
Cenanovic M. ; Kovačević, Lejla ; Pojskić, Naris ; Avdic J. ; Marjanović, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Journal of Human Genetics Vol 17: Supplement 2 / - : Nature Publishing Group, 2009, 281-281

Skup
The European Society of Human Genetics European Human Genetics Conference

Mjesto i datum
Beč, Austrija, 23.-26.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Y-STRs; population

Sažetak
In our previous population study, we have used twelve Y-chromosomal short tandem repeats loci incorporated in the PowerPlex®Y System to determine Y-STR diversity in B&H human population. With intent to obtain additional verification of the previously obtained results as well as to establish specific reference for a local B&H population, we have decided to test DNA samples collected from 100 unrelated healthy male Canton Sarajevo residents (from Sarajevo region) for the same twelve Y-linked short tandem repeats loci. Qiagen DnaeasyTM Tissue Kit was used for DNA extraction from buccal swabs and PowerPlex®Y System (Promega Corp., Madison, WI) has been used to simultaneously amplify Y-STR loci by PCR. PowerPlex®Y System includes 12 STR loci: DYS19, DYS385a, DYS385b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. The total PCR reaction volume was 5μl. PCR amplifications were carried out in PE GeneAmp PCR System Thermal Cycler. Electrophoresis of the amplification products was preformed on an ABI PRISM 310 genetic analyzer (ABI, Foster City, CA) according to the manufacturer's recommendations. The raw data were compiled and analyzed using the accessory software: ABI PRISM®Data Collection Software and Genemapper® v 3.2. In addition, we have compared the obtained “Sarajevo” dataset with the data previously generated for the entire Bosnian and Herzegovinian population, as well as with the available data on geographically close (neighboring) European populations. The results of this study will be used as guidelines in additional improving of research into genetic relationship among recent local B&H populations, both isolated and open, which is a long term project in our country

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Etnologija i antropologija



POVEZANOST RADA


Projekt / tema
309-1300855-2738 - Razvoj sustava za DNA analizu arheoloških koštanih uzoraka (Damir Marjanović, )

Ustanove
GENOS d.o.o.

Autor s matičnim brojem:
Damir Marjanović, (279190)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE