engleski

C-myc expression in the microvessels medulloblastomas

The increased expression of c-myc is related to neoplastic transformation and angiogenesis. Therefore the assessment of expression of c-myc in endothelial cells and neovascularization could help to determine the biological behavior of the tumor. We analyzed neovascularization and c-myc expression in 36 medulloblastoma specimens. Results were shown by determining immunohistochemical staining index (ISI), the sum of staining intensity (SI) and the percentage of reactive cells (PRC). We also performed the microvessel count (MVC) in 10 high-power fields (400X) with the most prominent vascularization and expressed it as microvessel density per mm2 (MVD). C-myc immunostaining intensity index in blood vessel endothelium is grouped in four groups, 0 – no reaction, I – weak reaction (ISI = 1 or 2), II – moderate reaction (ISI = 3 or 4), III – strong reaction (ISI = 5 or 6). Statistically significant differences (p=0.0214) have been found between groups 0 and 1 compared to groups 2 and 3. A higher percentage of reactive cells has been found in male patients than in female ones (p=0.0483). C-myc PRC 0 or 1 has on average smaller density of blood vessels per mm2 than c-myc PRC 2 or 3, but the difference is not statistically significant. C-myc ISI 0 or 1 has, on average, smaller density of blood vessels per mm2 than c-myc ISI 2 or 3, but the difference is not statistically significant. We conclude that c-myc staining intensity was connected with higher microvessels density.

c-myc; endothelium; medulloblastoma; microvessels; vascularization

nije evidentirano