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Pregled bibliografske jedinice broj: 461355

Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process


Trontel, Antonija; Baršić, Vanda; Slavica, Anita; Šantek, Božidar; Novak, Srđan
Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process // Food technology and biotechnology, 48 (2010), 3; 352-361 (međunarodna recenzija, članak, znanstveni)


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Naslov
Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process

Autori
Trontel, Antonija ; Baršić, Vanda ; Slavica, Anita ; Šantek, Božidar ; Novak, Srđan

Izvornik
Food technology and biotechnology (1330-9862) 48 (2010), 3; 352-361

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amylolytic lactic acid bacterium; Lactobacillus amylovorus; glucose; sucrose; starch; batch process; cultivation temperature; D/L-lactic acid; unstructured kinetic model; cardinal temperature model

Sažetak
Amylolytic lactic acid bacterium Lactobacillus amylovorus DSM 20531T utilised glucose, sucrose, and starch as a sole carbon and energy source. The three substrates were completely depleted from MRS medium during batch cultivations carried out in a lab-scale stirred tank bioreactor at constant temperature (40 C) and pH value (5.5). In tested conditions the bacterium was capable of conducting simultaneously starch hydrolysis and fermentation. A mixture of two stereoisomers, D-(-)- and L-(+)- lactic acid, was produced in all cases by highly efficient homofermentative bioprocess with 0.93-1 g lactate produced per g of total (consumed) substrate. The effect of temperature on the kinetics of cell growth and lactic acid production by the amylolytic strain in the starch containing medium was also investigated. Efficient simultaneous saccharification and fermentation (SSF) was obtained at 35, 40, and 45 °C with completely degraded complex carbohydrate over 8 - 12 h and with product yield coefficient in the range from 0.91 to 0.93 g/g. Maximum values for substrate consumption rate (0.89 h-1), maximum specific growth rate (0.87 h-1), product formation rate (2.01 h-1), and productivity of lactic acid (1.45 g/(L•h)) were obtained at 45 C, while maximum biomass concentration (4.38 g/L) was attained at 40 C. Ratio of the two stereoisomeric forms of produced lactic acid was strongly affected by the temperature. Unstructured kinetic model was used to describe consumption of the three substrates, bacterial biomass formation and lactic acid production by L. amylovorus DSM 20531T. The dependence of biokinetic parameters on temperature was described by cardinal temperature model. The applied models successfully predicted all experimental data.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
058-0581990-1997 - Primjena integriranih bioprocesa u proizvodnji mliječne kiseline (Srđan Novak, )
058-0581990-2004 - Hibridni integrirani bioprocesi i održivost proizvodnje organskih otapala (Božidar Šantek, )

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Anita Slavica (autor)

Avatar Url Srđan Novak (autor)

Avatar Url Božidar Šantek (autor)

Avatar Url Antonija Trontel (autor)

Citiraj ovu publikaciju

Trontel, Antonija; Baršić, Vanda; Slavica, Anita; Šantek, Božidar; Novak, Srđan
Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process // Food technology and biotechnology, 48 (2010), 3; 352-361 (međunarodna recenzija, članak, znanstveni)
Trontel, A., Baršić, V., Slavica, A., Šantek, B. & Novak, S. (2010) Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process. Food technology and biotechnology, 48 (3), 352-361.
@article{article, year = {2010}, pages = {352-361}, keywords = {amylolytic lactic acid bacterium, Lactobacillus amylovorus, glucose, sucrose, starch, batch process, cultivation temperature, D/L-lactic acid, unstructured kinetic model, cardinal temperature model}, journal = {Food technology and biotechnology}, volume = {48}, number = {3}, issn = {1330-9862}, title = {Modeling the Effect of Different Substrates and Temperature on the Growth and Lactic Acid Production by Lactobacillus amylovorus DSM 20531T in Batch Process}, keyword = {amylolytic lactic acid bacterium, Lactobacillus amylovorus, glucose, sucrose, starch, batch process, cultivation temperature, D/L-lactic acid, unstructured kinetic model, cardinal temperature model} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus