engleski

Metabolic changes in hepatocytes from rats on raw soybean diet

Background and aims: A high level of dietary fat intake is believed to be a major factor in the development of obesity and insulin resistance. Low carbohydrate /high n-6 polyunsaturated fatty acids (PUFA) diet causes the development of insulin resistance, without development of obesity. The aim of this study was to determine metabolic changes and insulin effects in vivo and in vitro in rats fed three weeks with raw soybean containing high level n-6 and n-3 PUFA. Materials and methods: Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h. 14C-U-glucose incorporation in glycogen, alfa-amino14C-isobutyric acid (AIB) transport and glucose production was measured. Results: Intravenous glucose tolerance test was normal, but basal glucose production was 50% higher in hepatocytes isolated from rats on a raw soybean diet. The glucagon 146 % increased glucose production in hepatocytes from rats on standard diet. Glucagon effect was 86 % higher in hepatocytes from rats on raw soybean and insulin did not decrease basal or glucagon-stimulated glucose production. In hepatocytes from rats on standard diet insulin produced more 100% increase of glucose incorporation into glycogen. In rats on raw soybean diet basic glucose incorporation was very low and insulin did not change rate of glucose incorporation. In hepatocytes obtained from rats on raw soybean diet basic AIB transport was 37% higher, but insulin effect was strongly reduced. Conclusion: These results clearly demonstrate that altering the macronutrient proportion of the standard diet from high carbohydrate/low fat to high n-6, n-3 PUFA/low carbohydrate diet causes the development of insulin resistance with negative energy balance.

3 and n-6 PUFA) ; hepatocytes ; insulin ; insulin resistance

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