engleski

Comparison of cell proliferation in canine and feline mammary gland hyperplasia and neoplasia

INTRODUCTION Ki-67 nuclear antigen is one of the main immunohistochemical (IHC) marker used to evaluate tumor proliferative activity. Studies on canine mammary tumors have revealed an association between proliferative activity and several clinical and pathological variables but similar studies on feline mammary tumors produced contradictory results. The purpose of this study was to investigate and to compare the IHC expression of the Ki-67 antigen in the canine and feline mammary hyperplasia, benign and malignant tumors. MATERIALS AND METHODS Seventy-five canine and 23 feline surgical specimens of mammary tumors were studied. Tumors were classified according to the World Health Organisation and histological grading of carcinomas was performed according to the Nottingam method. IHC determination of Ki-67 was performed using the streptavidin-biotin-peroxidase complex method. RESULTS Comparison between proliferative activity in different histological types of canine mammary tumors showed that the Ki-67 indeks was significantly higher in anaplastic than in tubulopapillary carcinomas (p0, 05). Comparison of proliferative activity and histological grade of carcinoma showed that the Ki-67 indeks was significantly higher in moderate and poor differentiated carcinomas than in benign and well differentiated tumors (p0, 05). In feline mammary carcinomas the Ki-67 indeks was significantly higher in poor differentiated carcinomas than in benign, well and moderate differentiated tumors (p0, 05). There were no association between Ki-67 expression and presence of necroses or tumor infiltrates. CONCLUSION Ki-67 represents a robust marker of cell proliferation in routinly processed canine and feline mammary tumors. In both species, the Ki-67 index correlate with tumor grade, but only in canine mammary tumors the Ki-67 indeks correlate also with different tumor type.

mammary; neoplasia; Ki67; dog; cat

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