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Pregled bibliografske jedinice broj: 455584

Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population


Banović, Miroslav; Bordukalo-Nikšić, Tatjana; Balija, Melita; Čičin-Šain, Lipa; Jernej, Branimir
Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population // Platelets, 21 (2010), 6; 429-438 doi:10.3109/09537104.2010.487582 (međunarodna recenzija, članak, znanstveni)


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Naslov
Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population

Autori
Banović, Miroslav ; Bordukalo-Nikšić, Tatjana ; Balija, Melita ; Čičin-Šain, Lipa ; Jernej, Branimir

Izvornik
Platelets (0953-7104) 21 (2010), 6; 429-438

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
serotonin transporter; 5-HTt; SERT; blood platelets; physiology; kinetic analysis

Sažetak
The present study had two goals: first, to give detailed description of the reliable method for full kinetic analysis of serotonin transporter (5HTt) on the membrane of human platelets, and second, as a main issue, to report on fundamental physiological characteristics of this transmembrane transport on a large population of healthy individuals. Full-kinetics analysis of platelet serotonin uptake were performed on 334 blood donors of both sexes by the use of 14C-radioisotopic method, which was first optimized according assumptions of enzyme kinetic analyses, with regard to platelet concentration, duration of uptake, concentration of substrate as well as important technical parameters (underpressure of filtration, blanks, incubating temperature etc). Kinetic parameters of platelet serotonin uptake in the whole population were for Vmax: 142  25.3 pmol 5HT/108 platelets/minute and for Km: 0.404  0.089 M 5HT. Besides report on kinetic values of 5HT transporter protein, we have also described fundamental physiological characteristics of Vmax, Km and their derivative, Vmax/Km (transporter efficiency): range and frequency distribution of normal values, intraindividual stability over time, lack of age influence, gender dependence and seasonal variations. The report on kinetic values and main physiological characteristics of serotonin transport into platelets, obtained by the use of thoroughly reassessed methodology, and on by far the largest human population studied until now, offers a reliable frame of reference for pathophysiological studies of this parameter in various clinical fields.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-1081870-2395 - Serotonergična neurotransmisija: geni, proteini i ponašanje (Stefulj, Jasminka, MZOS - )
098-1081870-2397 - Serotonergični mehanizmi u alkoholizmu (Čičin-Šain, Lipa, MZOS - )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Banović, Miroslav; Bordukalo-Nikšić, Tatjana; Balija, Melita; Čičin-Šain, Lipa; Jernej, Branimir
Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population // Platelets, 21 (2010), 6; 429-438 doi:10.3109/09537104.2010.487582 (međunarodna recenzija, članak, znanstveni)
Banović, M., Bordukalo-Nikšić, T., Balija, M., Čičin-Šain, L. & Jernej, B. (2010) Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population. Platelets, 21 (6), 429-438 doi:10.3109/09537104.2010.487582.
@article{article, year = {2010}, pages = {429-438}, DOI = {10.3109/09537104.2010.487582}, keywords = {serotonin transporter, 5-HTt, SERT, blood platelets, physiology, kinetic analysis}, journal = {Platelets}, doi = {10.3109/09537104.2010.487582}, volume = {21}, number = {6}, issn = {0953-7104}, title = {Platelet serotonin transporter (5HTt) : physiological influences on kinetic characteristics in a large human population}, keyword = {serotonin transporter, 5-HTt, SERT, blood platelets, physiology, kinetic analysis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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