engleski

Maize seryl-tRNA synthetase: specificity of substrate recognition by the organellar enzyme

In our study of seryl-tRNA formation in maize, we characterized the enzymes involved in serylation. Only two dissimilar seryl-tRNA synthetase (SerRS) cDNA clones were identified in the Zea mays EST databases. One encodes a seryl-tRNA synthetase which presumably functions in the organelles, while the other synthetase product is more similar to eukaryotic cytosolic counterparts. We analyzed the specificity and the mechanism of substrate recognition by purified overexpressed organellar SerRS. This enzyme displays tRNA-dependent serine activation and aminoacylates maize mitochondrial and chloroplast tRNASer transcripts with similar efficiences, which may suggest that only two isoforms of seryl-tRNA synthetase are sufficient to catalyze seryl-tRNASer formation in three cellular compartments of Zea mays.

seryl-tRNA synthetases ; organelles ; Zea mays ; tRNA-dependent amino acid recognition ; horizontal gene transfer

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