engleski

Effect of azithromycin on acute inflammatory lesions and colonic bacterial load in a murine model of experimental colitis

The aim of this study was to investigate the effect of the macrolide antibiotic azithromycin on mucosal changes and colonic bacterial load in u murine model of colitis. Colitis was induced in CD1 mice using enema of 0, 2% solution of dinitrofluorobenzene, combined with skin sensitization.Four experimental groups of animals were treated with azithromycin and/or metronidazole. Clinical and histopathological scores were used to assess inflammatory changes, and colon washings were used to determine changes in bacterial load. The anti-inflammatory effect of azithromycin did not differ from the effect of methylprednisolone. Number of colonic bacteria did not differ significantly between control and experimental groups of animals. We documented the anti-inflammatory effect of azithromycin in a murine model of acute colitis, suggesting the effects targeted to oxidative burst and on mucosal/bacterial interface, independent of luminal bacterial load.

inflammatory bowel disease; experimental colitis; inflammation; azithromycin; bacterial biofilm

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