engleski

Ergometry Induces Systemic Oxidative Stress in Healthy Human Subjects

Oxidative stress, i.e. excess in production of oxygen free radicals, is important pathogenic factor of cancer and cardiovascular, metabolic and degenerative diseases. On the other hand, mild oxidative stress, as in case of physical exercise, can increase the antioxidant defense system. However, the mechanisms underlying such desirable effects of mild oxidative stress are not well understood, in particular because the production of the most important oxygen free radical, hydroxyl radical, was not yet evaluated under physiological circumstances. Therefore, in this study we determined hydroxyl radical generation in the blood of ten healthy volunteers before and after ergometry, by trapping it with salicylic acid and analyzing the reaction specific product, 2, 3-dihydroxybenzioc acid, using high performance liquid chromatography. Oxidative stress response to exercise was also evaluated by measuring total serum peroxides and total antioxidant capacity of serum. These parameters of oxidative stress were then correlated with physical performance of the subjects. Ergometry caused an increase of the hydroxyl radical level by 37.5% (p < 0.05). The levels of total serum peroxides did not change significantly after ergometry. Total serum antioxidant capacity was significantly higher after ergometry by 39.7% (p < 0.05), and was in positive correlation (r=0.81) with anaerobic threshold, which is reliable indicator of physical condition. Hence, we may conclude that ergometry induces hydroxyl radical production and systemic oxidative stress response mobilizing antioxidants in healthy humans, proportional to their physical condition. Therefore, ergometry could be used to study physiological oxidative stress response and to improve antioxidant defense capacities of the organism.

hydroxyl radical ; serum peroxides ; antioxidants ; oxidative homeostasis ; ergometry

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