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Pregled bibliografske jedinice broj: 446731

Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish


Sauerborn Klobučar, Roberta; Žaja, Roko; Franjević, Damjan; Brozović, Anamaria; Smital, Tvrtko
Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish // Arhiv za higijenu rada i toksikologiju, 61 (2010), 2; 175-181 (međunarodna recenzija, članak, znanstveni)


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Naslov
Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish

Autori
Sauerborn Klobučar, Roberta ; Žaja, Roko ; Franjević, Damjan ; Brozović, Anamaria ; Smital, Tvrtko

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 61 (2010), 2; 175-181

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aquatic organisms ; multixenobiotic resistance (MXR) ; ABC proteins ; Western blot analysis ; Phylogenetic analysis

Sažetak
One of the most intriguing defense strategies evolutionary developed in aquatic organisms is the activity of the multixenobiotic resistance (MXR) mechanism. Analogous to the multidrug resistance (MDR) phenomenon expressed in various mammalian tissues, it has been shown that ABC transport proteins are the key mediators of MXR activity. They provide resistance of aquatic organisms by binding xenobiotics and extruding them from cells in an energy-dependent manner. Since Cyprinid fish species are common target in freshwater biomonitoring programs, we have studied the presence of two main MDR/MXR efflux transporters: the P-glycoprotein (Pgp, Abcb1) and the MRP-like protein(s) (Abcc), in liver tissue from five Cyprinid fish species: common carp - Cyprinus carpio ; European chub - Sqalius cephalus ; sneep - Chondostroma nasus ; barbel – Barbus barbus ; and silver prussian carp – Carassius auratus gibelio. Their presence was evaluated on the mRNA and protein level. Various pairs of primers were designed to clone homologous fragments of MXR-related genes. Evaluation at the protein level was done by Western blotting using specific monoclonal antibodies raised against the human Pgp (Abcb1, Ab C219), MRP1 (Abcc1 ; Ab MRPm6) or MRP2 (Abcc2 ; Ab M2I-4). Transcripts of both key types of MXR transporters were identified in all species examined and here we provide the phylogenetic analysis for new partial sequences obtained. Immunochemical determinations with mammalian antibodies failed to identify the presence of MRP(s), but the Pgp expression was found in all five Cyprinid species. These results support the general idea about MXR as a defense system mediated by both Pgp and MRP types of ABC transport proteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
04M3109
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Tvrtko Smital, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Citiraj ovu publikaciju

Sauerborn Klobučar, Roberta; Žaja, Roko; Franjević, Damjan; Brozović, Anamaria; Smital, Tvrtko
Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish // Arhiv za higijenu rada i toksikologiju, 61 (2010), 2; 175-181 (međunarodna recenzija, članak, znanstveni)
Sauerborn Klobučar, R., Žaja, R., Franjević, D., Brozović, A. & Smital, T. (2010) Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish. Arhiv za higijenu rada i toksikologiju, 61 (2), 175-181.
@article{article, year = {2010}, pages = {175-181}, keywords = {aquatic organisms, multixenobiotic resistance (MXR), ABC proteins, Western blot analysis, Phylogenetic analysis}, journal = {Arhiv za higijenu rada i toksikologiju}, volume = {61}, number = {2}, issn = {0004-1254}, title = {Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish}, keyword = {aquatic organisms, multixenobiotic resistance (MXR), ABC proteins, Western blot analysis, Phylogenetic analysis} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • SCI Expanded
  • Animal Science Database
  • GreenFile
  • INIS
  • Veterinary Science Database