engleski

Biotechnology of human skin cell cultivation - logistic aspects and infrastructural support

Extensive skin defects after burns are covered by autologous or allogeneic skin transplants, lyophilized animal skin, artificial tissues made of biodegradable material or skin epithelial cells (keratinocytes) cultured in vitro. Autologous keratinocytes obtained from a skin biopsy may in three weeks yield progeny sufficient to cover a large body area of a burned patient. The period required for the expansion of the keratinocyte cultures is bridged by temporary transplants of allogeneic skin or artificial material. Cultivation of human keratinocytes has been started as a pilot project in our Institute and the first cultures have been obtained. So far the cultures were used for the research purposes. Clinical application of cultured keratinocytes is planned to take place in collaboration with plastic surgeons. Logistic prerequisites for the production and clinical use of cultured keratinocytes include: appropriate laboratory space and equipment, specifically educated laboratory personnel, safety testing of the cultured material, ethical committee permission for clinical testing of the cultured material, a trained surgical and supportive care team, and financial background.

human keratinocytes; cells cultured; biotechnology; burns

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