engleski

Human papillomavirus infection in men: prevalence and HPV type distribution

Background:Human papillomavirus (HPV) infection has been identified as a major risk factor for cervical and other anogenital cancers. Although HPV can infect women and men, little information is available regarding HPV infection in men, its usually presented as subclinical, with large number of asymptomatic carriers. Objectives: We have performed this study in order to better understand natural history of HPV infection in men, especially the prevalence of HPV DNA and HPV type distribution. Methods: In last three years, we have tested 581 men from Zagreb and Rijeka, 18 to 46 years old for HPV DNA at the penile surface, including glans/coronal sulcus and penile shaft, and distal urethral canal. The samples containing exfoliated epithelial cells were collected by saline-wetted cotton swabs placed into a specimen transport medium (Digene Corp., Gaithersburg, MD). DNA was isolated by Nucleospin Tissue isolation kit ( Macherey-Nagel GmbH, Duren, Germany). HPV DNA detection and genotyping was performed by consensus and type-specific primers directed PCR (Takara, Japan, Gravitt et al. 2000, andWalboomers et al. 1999) including low-risk HPV (6, 11) and high-risk HPV (type specific amplification:16, 18, 31, 33 and unclassified hr-HPVtypes). Results: The overall prevalence ofHPVDNApositive men High-riskHPV(hrHPV) was detected in 14.11%(82/581) and lowriskHPVin 13.77% (80/581) cases, respectively including glans/coronal sulcus and penile shaft with 25.26% (99/392), compared to 9.83% (29/295) in the urethra (Figure 2).. Out of hrHPV positive specimens, HPV 16 was the predominant type found in 39.02% cases, followed by unclassified HPV type (29.27%), HPV 18 (12.19%), HPV 31 (9.75%) and HPV 33 (9.75%) (Figure 3). HPV infection with multipleHPVtypes occurs in 15.72% (25/159) cases (Figure 4).

HPV; HPV infection; men; HPV genotype

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