engleski

Tissue microarray study of tumour biomarkers in HPV induced cervical neoplasia.

The aim of this study was to analyse expression patterns of tumour biomarkers Ki 67, p53, p16 (INK4A) and epidermal growth factor receptor (EGFR), in cervical carcinoma using tissue microarray technology(TMA). Methods: The expression of Ki67, p53, p16(INK4A) and EGFR was studied in series of 34 cervical cancer samples: 6 cervical intraepithelial neoplasia (CIN III), 14 squamous cell carcinomas (SCC), and 14 adenocarcinomas (ADC). TMA slides containing triplicate of 1.0 mm cores were constructed and subjected to immunohistochemical protein analysis. Results: High-risk HPV-DNA was identified in 94 % (32/34) of the cases. HPV-16 predominated in SCC and CIN III, while HPV 18 and HPV 16/18 mixed infection were detected in ADC. Ninety-one percent of samples (31/34) exhibited strong proliferative activity (+2 and +3 Ki 67 immunopositivity), 64% (22/34) exhibited high expression of p16(INK4A), 73% (25/34) demonstrated high expression of p53 and 61 % (21/34) of uterine cervix neoplasia exhibited high EGFR expression. According to the histological type, SCC and ADC demonstrated high expression of Ki67, p16(INK4A) and p53. In CIN III only high expression was detected for Ki67, while p16(INK4A) and p53 were expressed at low level. Substantial differences were shown in EGFR expression: SCC and CIN III demonstrated high level of EGFR expression, in 92 % (13/14) and 100% (6/6) samples, respectively. In eighty-five percent of ADC’s the expression of EGFR was absent (9/14) or (+1) intensity (3/14). Only two tumours demonstrated moderate/strong (+2) level of expression. Conclusion: Our study has demonstrated the reliability of tissue microarray technology in protein expression studies. Preliminary data revealed substantial differences in tumour markers expression in different histological types of cervical neoplasia. To confirm these results, larger studies are needed. Especially, EGFR expression in ADC and adenosquamous carcinoma should be investigated to elucidate the role of EGFR expression in these histological types of cervical carcinomas.

Cervical Neoplasia; HPV; Tissue Microarray

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