engleski

Dendritic cells at the maternal fetal interface

Dendritic cells (DCs) are able to balance between inflammation and tolerance and therefore could be involved in the implantation processes and regulation of trophoblast invasion. There is no unique concept about the characteristics of distinct DCs subsets which differentially bias NK and T cell response. Beside foreign antigens, it is considered that local microenvironment, are potent modulators of DCc function. Epithelial cells product, Tumor Associated Glycoprotein-72 (TAG-72) is physiologically presented in secretory phase human endometrium, but negative immunostaining of TAG-72 was seen in uterine deciduas of normal first trimester pregnancies. The importance of TAG-72 removal in early pregnancy and its possible immunological role still remain obscure. In vitro experiments showed that decidual CD1a+ cells are able to bind and internalize TAG-72 by carbohydrate recognition domain of CD206 and CD209 endocytic receptors. TAG-72 decreased CD83 and IFN- expressions in CD1a+ cells disabling them to settle decidual T cells toward pro-inflammatory orientation. Down-regulation of IL-15 and IL-18 cytokines expression in TAG-72 treated CD1a+ cells hampered the NK cell proliferation in a close contact with TAG-72 treated CD1a+ cells. In ectopic pregnancy TAG-72 was found by immunohistology in tubal mucosa close to the implantation site and away from the implantation site, but not in the uterine decidua. The percentage of IL-15+ cells and NK cells were drastically reduced at the mucosal sites characterized with the appearance of TAG- 72. All these results suggest that TAG-72, if present at the implantation site could hamper physiological mild pro-inflammatory response and NK cells proliferation, which could lead to inadequate trophoblast growth control. Acknowledgement: The experiments were financed by the Grants of Croatian Ministry of Science, Education and Sports No. 0620402-0376, No. 062- 620402-0377 and No. 0620402-0379.

Dendritic cells; TAG-72; early pregnancy

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