Effect of low dose gamma-radiation upon antioxidant enzymes in chick embryo liver (CROSBI ID 158598)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vilić, Marinko ; Aladrović, Jasna ; Beer Ljubić, Blanka ; Miljanić, Saveta ; Kraljević, Petar
engleski
Effect of low dose gamma-radiation upon antioxidant enzymes in chick embryo liver
Exposure of rodents to low-dose whole-body irradiation could cause various antioxidant mechanisms that defend cells from oxidative stress. However, there is a lack of results on antioxidant status in organs of chicken after low dose eggs or whole-body irradiation. This study was performed to investigate the effect of low-dose gamma-irradiation upon activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and level of glutathione (GSH) and lipid peroxidation (TBARS) in liver of chick embryo and newly hatched chicks. Fertilized chicken eggs were irradiated with the dose of 0.3 Gy gamma radiation (source 60Co) on the 19th day of incubation. The liver samples were taken on 6, 24, 48, 72 and 96 h after egg irradiation. The antioxidant parameters were measured spectrophotometrically. Level of GSH and lipid peroxidation as well GSH-Px activity increased in the liver of chick embryos on the 6th hour after irradiation. GSH-Px and SOD activity significantly decreased in the liver of chick embryo on the 48 h after egg exposure. Lipid peroxidation level and GSH level also significantly decreased in the liver of chick embryo on the 48 h after egg exposure. CAT activity during the experiment was not statistically different when it compared to controls. These results suggest that chick embryo exposure to dose of 0.3 Gy gamma-radiation on the 19th day of incubation causes alteration of antioxidant status in chick embryo liver. It also seems, as is the case in mammals, that GSH and GSH-Px could be the first choice in antioxidant defence in chicken embryo liver at early time after low dose irradiation.
Chick embryo; gamma-radiation; antioxidative enzymes; liver
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano