engleski

Comparison of Cytotoxicity Induced by 17α-Ethynylestradiol and Diethylstilbestrol in Fish CCO and Mammalian CHO-K1 Cell Lines

The effects of synthetic estrogens 17α-ethynylestradiol (EE2) and diethylstilbestrol (DES) were compared on cell proliferation and morphology in Channel Catfish Ovary (CCO) and Chinese Hamster Ovary (CHO-K1) cells. EE2 exposure (0.1 and 0.5 µg/mL) induced stimulatory effect on CCO and CHO-K1 cell proliferation, while higher concentrations (1-10 µg/mL) showed cytotoxic effects. Increase in DES concentrations mainly resulted in dose-depended increase in cytotoxicity in both cell lines. Morphological changes induced by EE2 and DES exposure (5µg/mL) showed disrupted cell monolayer and increased number and size of lysosomes. Comparison of IC50 values showed almost equal sensitivity towards cytotoxicity of tested compounds in CCO and CHO-K1 cells.

CCO cells; CHO-K1 cells; Cytotoxicity; Synthetic estrogens

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