engleski

Stimulation of short-circuit current across lobster (Homarus americanus) epipodite by sinus gland extract

In lobsters acclimated to dilute seawater, short- circuit current (Isc) was measured before and after basolateral application of sinus gland extract (SGE) prepared by homogenizing two homologous sinus glands in cold saline and diluting to 6 ml. Isc was significantly stimulated by SGE from -91.6􀁲 6.2 􀁐A cm-2 to -143.9􀁲6.4 (P<0.01 ; Figure 1), with no lag period. Following removal of SGE, Isc approached control values within 50 minutes, indicating that the effect of SGE is reversible. The degree of stimulation was shown to be dosedependent. Pretreatment of SGE with proteinase K blocked the stimulatory effect, leading to our conclusion that the active factor is proteinaceous.

lobster; sinus gland; short cirrcuit current

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