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Reversed-Phase HPLC Separation of C2 and C3 Isomers of 7-ADCA and Cephalexin Monohydrate

High-performance liquid chromatography was applied for the separation and determination of C2 and C3 isomers of 7-aminodesacetoxycephalosporanic acid (7-ADCA) and cephalexin monohydrate in their mixtures. Separation was performed on a column containing bonded octadecyl silica phase. The effects of pH (in the range of 3.0-7.6) on the investigated compounds were studied. Two organic modifiers, acetonitrile and methanol, were used to improve and accelerate separation. The applied procedure was very reliable for quantitative determination. Exellent corellation with the data of microbiological assay was found. The procedure was very convenient even when other impurities were present in the sample. This is an advantage with respect to microbiological assays.

isomers of 7-ADCA ; HPLC separation

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