engleski

Altered glycosphingolipid expression in mice lacking TNF receptor 1

Expression of neutral glycosphingolipids (GSLs) and gangliosides in normal lymphoid tissues and cells has been studied mostly by biochemical and immunochemical analysis of lipid extracts separated by thin-layer chromatography. GSLs and gangliosides involved in GM1b-biosynthetic pathway were assigned to T lymphocytes, whereas B cell gangliosides and GSLs have been poorly characterized by former publications. We used specific polyclonal antibodies in immunohistology and flow cytometry to analyze the distribution of globotriaosylceramide (Gb3Cer), globoside (Gb4Cer), gangliotriaosylceramide (Gg3Cer), gangliotetraosylceramide (Gg4Cer), and gangliosides GM3 and GalNAc-GM1b in the mouse thymus, spleen, and lymph node from normal mice and mice homozygous for a gene knockout for TNFRp55.

nije evidentirano

nije evidentirano