In vitro multile shoot induction and plant regeneration in Betula pendula (CROSBI ID 158071)
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Besendorfer, Višnja ; Kolevska-Pletikapić, Branka
engleski
In vitro multile shoot induction and plant regeneration in Betula pendula
Shoot development and plant regeneration through axillary bud culture of the European birch (Betula pendula Roth.) were successfully achieved. The modified ACM medium supplemented with 2.2 uM BA was used as initial medium. Both adventitious and axillary shoot development was induced in 2-year-old genotype explants, while in 4-year-old genotype cultures only axillary shoots developed. The establishment of multiple shoot culture was genotype depending. It was found that ACM nutrition medium, supplemented with 2.2 uM BA, was more effective for shoot multiplication than WPM and BTM. Microcuttings were easily rooted on ACM medium supplemented with 1.0 uM IBA. The complete plants obtained were successfully transferred to soil.
european birch; micropropagation
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