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izvor podataka: crosbi

Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia Holbrooki) Inhabiting an Aluminium-Polluted Water Environment: an Alkaline Comet Assay Study (CROSBI ID 158060)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Ternjej, Ivančica ; Mihaljević, Zlatko ; Stanković, Igor ; Kerovec, Mladen ; Sipos, Laszlo ; Želježić, D. ; Kopjar, Nevenka Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia Holbrooki) Inhabiting an Aluminium-Polluted Water Environment: an Alkaline Comet Assay Study // Archives of environmental contamination and toxicology, 59 (2010), 2; 182-193. doi: 10.1007/s00244-010-9469-6

Podaci o odgovornosti

Ternjej, Ivančica ; Mihaljević, Zlatko ; Stanković, Igor ; Kerovec, Mladen ; Sipos, Laszlo ; Želježić, D. ; Kopjar, Nevenka

engleski

Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia Holbrooki) Inhabiting an Aluminium-Polluted Water Environment: an Alkaline Comet Assay Study

To estimate the impacts of an Al-contaminated aquatic environment on DNA integrity in the blood cells of eastern mosquitofish Gambusia holbrooki Girard 1859 inhabiting Lake Njivice (Island of Krk, Croatia), an evaluation using the alkaline comet assay was carried out. Genome integrity was studied in parallel with the same fish species inhabiting the nearby, unpolluted Lake Ponikve. The amount of DNA damage in cells was estimated from three different parameters: comet tail length as the extent of genetic material migration, tail intensity (% DNA in the comet tail) and tail moment. The results indicate the loss of genome integrity in blood cells of mosquitofish inhabiting Lake Njivice and the genotoxicity of this aquatic environment. Using the same assay, acute genotoxicity of contaminated water and sediment was evaluated and confirmed on fish, mouse and human blood cells treated ex vivo. Results of the present study indicate that the alkaline comet assay applied to fish blood cells is a valuable tool for determining the potential genotoxicity of water pollutants and confirm its usefulness in the evaluation of DNA damage in fish living in Al-polluted waters.

mosquitofish; DNA integrity; comet assay; aluminium; sediment; genotoxicity

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

59 (2)

2010.

182-193

objavljeno

0090-4341

1432-0703

10.1007/s00244-010-9469-6

Povezanost rada

Biologija

Poveznice
Indeksiranost