Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 439418

Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry


Kazazić, Saša; Zhang, Hui-Min; Schaub, Tanner M.; Emmett, Mark R.; Hendrickson, Christopher L.; Blakney, Gregory T.; Marshall, Alan G.
Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry // Journal of the American Society for Mass Spectrometry, 21 (2010), 4; 550-558 doi:10.1016/j.jasms.2009.12.016 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 439418 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry

Autori
Kazazić, Saša ; Zhang, Hui-Min ; Schaub, Tanner M. ; Emmett, Mark R. ; Hendrickson, Christopher L. ; Blakney, Gregory T. ; Marshall, Alan G.

Izvornik
Journal of the American Society for Mass Spectrometry (1044-0305) 21 (2010), 4; 550-558

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptide ; protein ; H/D exchange ; autosampler ; mass spectrometry ; ICR ; ESI ; FT-ICR ; FTMS ; LTQFT

Sažetak
Mass analysis of proteolytic fragment peptides following hydrogen/deuterium exchange offers a general measure of solvent accessibility/hydrogen bonding (and thus conformation) of solution-phase proteins and their complexes. The primary problem in such mass analyses is reliable and rapid assignment of mass spectral peaks to the correct charge state and degree of deuteration of each fragment peptide, in the presence of substantial overlap between isotopic distributions of target peptides, autolysis products, and other interferant species. Here, we show that at sufficiently high mass resolving power (m/delta m50% > 100, 000), it becomes possible to resolve enough of those overlaps that automated data reduction becomes possible, based on the actual elemental composition of each peptide without the need to deconvolve isotopic distributions. We demonstrate automated, rapid, reliable assignment of peptide masses from H/D exchange experiments, based on electrospray ionization FT-ICR mass spectra from H/D exchange of solution-phase myoglobin. Combined with previously demonstrated automated data acquisition for such experiments, the present data reduction algorithm enhances automation (and thus expanded generality and applicability) for high-resolution mass spectrometry-based analysis of H/D exchange of solution-phase proteins.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
098-0982915-2945 - Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula (Kovač, Branka, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Saša Kazazić (autor)

Poveznice na cjeloviti tekst rada:

doi link.springer.com doi.org

Citiraj ovu publikaciju:

Kazazić, Saša; Zhang, Hui-Min; Schaub, Tanner M.; Emmett, Mark R.; Hendrickson, Christopher L.; Blakney, Gregory T.; Marshall, Alan G.
Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry // Journal of the American Society for Mass Spectrometry, 21 (2010), 4; 550-558 doi:10.1016/j.jasms.2009.12.016 (međunarodna recenzija, članak, znanstveni)
Kazazić, S., Zhang, H., Schaub, T., Emmett, M., Hendrickson, C., Blakney, G. & Marshall, A. (2010) Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry. Journal of the American Society for Mass Spectrometry, 21 (4), 550-558 doi:10.1016/j.jasms.2009.12.016.
@article{article, author = {Kazazi\'{c}, Sa\v{s}a and Zhang, Hui-Min and Schaub, Tanner M. and Emmett, Mark R. and Hendrickson, Christopher L. and Blakney, Gregory T. and Marshall, Alan G.}, year = {2010}, pages = {550-558}, DOI = {10.1016/j.jasms.2009.12.016}, keywords = {peptide, protein, H/D exchange, autosampler, mass spectrometry, ICR, ESI, FT-ICR, FTMS, LTQFT}, journal = {Journal of the American Society for Mass Spectrometry}, doi = {10.1016/j.jasms.2009.12.016}, volume = {21}, number = {4}, issn = {1044-0305}, title = {Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry}, keyword = {peptide, protein, H/D exchange, autosampler, mass spectrometry, ICR, ESI, FT-ICR, FTMS, LTQFT} }
@article{article, author = {Kazazi\'{c}, Sa\v{s}a and Zhang, Hui-Min and Schaub, Tanner M. and Emmett, Mark R. and Hendrickson, Christopher L. and Blakney, Gregory T. and Marshall, Alan G.}, year = {2010}, pages = {550-558}, DOI = {10.1016/j.jasms.2009.12.016}, keywords = {peptide, protein, H/D exchange, autosampler, mass spectrometry, ICR, ESI, FT-ICR, FTMS, LTQFT}, journal = {Journal of the American Society for Mass Spectrometry}, doi = {10.1016/j.jasms.2009.12.016}, volume = {21}, number = {4}, issn = {1044-0305}, title = {Automated data reduction for hydrogen/deuterium exchange experiments, enabled by high-resolution Fourier transform ion cyclotron resonance mass spectrometry}, keyword = {peptide, protein, H/D exchange, autosampler, mass spectrometry, ICR, ESI, FT-ICR, FTMS, LTQFT} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font