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Pregled bibliografske jedinice broj: 437781

Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)


Lončar, Jovica; Popović, Marta; Žaja, Roko; Smital, Tvrtko
Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss) // Comparative biochemistry and physiology. C. Toxicology & pharmacology, 151 (2010), 2; 209-215 doi:10.1016/j.cbpc.2009.10.009 (međunarodna recenzija, članak, znanstveni)


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Naslov
Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)

Autori
Lončar, Jovica ; Popović, Marta ; Žaja, Roko ; Smital, Tvrtko

Izvornik
Comparative biochemistry and physiology. C. Toxicology & pharmacology (1532-0456) 151 (2010), 2; 209-215

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ABC efflux transporters ; xenobiotics ; rainbow trout ; mRNA expression ; tissue distribution

Sažetak
In this study we examined gene expression of a series of ABC efflux transporters in various rainbow trout (Oncorhynchus mykiss) tissues. Based on their reported toxicological relevance, we have used quantitative real time PCR SYBR green quantification methodology, with combination of absolute and relative approach, to quantify RNA expression of eight ABC transporters from three different families: abcb1 and abcb11, abcc1-5 and abcg2. Level of mRNA transcripts was measured in seven tissues: liver, brain, gonads, kidney, gills, proximal intestine and distal intestine, and the obtained expression profiles were compared with data available for related mammalian tissues. Most of the analyzed genes showed similar gene expression pattern as the ones found in mammals, with some notable exceptions. E.g., gills were in our study characterized by extremely low expression of all analyzed ABC genes ; and despite the pronounced role of ABCC1 (MRP1) in protection of mammalian cells from chemical toxins, we found low expression of this transporter in trout tissues. Taken together, our study offers the first thorough insight into distribution pattern of (eco)toxicologically relevant ABC transporters, serving as a necessary base for further studies directed to better understanding of physiological and/or protective role of ABC transporters in fish.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Smital, Tvrtko, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Roko Žaja (autor)

Avatar Url Jovica Lončar (autor)

Avatar Url Tvrtko Smital (autor)

Avatar Url Marta Popović (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com doi.org

Citiraj ovu publikaciju:

Lončar, Jovica; Popović, Marta; Žaja, Roko; Smital, Tvrtko
Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss) // Comparative biochemistry and physiology. C. Toxicology & pharmacology, 151 (2010), 2; 209-215 doi:10.1016/j.cbpc.2009.10.009 (međunarodna recenzija, članak, znanstveni)
Lončar, J., Popović, M., Žaja, R. & Smital, T. (2010) Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss). Comparative biochemistry and physiology. C. Toxicology & pharmacology, 151 (2), 209-215 doi:10.1016/j.cbpc.2009.10.009.
@article{article, year = {2010}, pages = {209-215}, DOI = {10.1016/j.cbpc.2009.10.009}, keywords = {ABC efflux transporters, xenobiotics, rainbow trout, mRNA expression, tissue distribution}, journal = {Comparative biochemistry and physiology. C. Toxicology and pharmacology}, doi = {10.1016/j.cbpc.2009.10.009}, volume = {151}, number = {2}, issn = {1532-0456}, title = {Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)}, keyword = {ABC efflux transporters, xenobiotics, rainbow trout, mRNA expression, tissue distribution} }
@article{article, year = {2010}, pages = {209-215}, DOI = {10.1016/j.cbpc.2009.10.009}, keywords = {ABC efflux transporters, xenobiotics, rainbow trout, mRNA expression, tissue distribution}, journal = {Comparative biochemistry and physiology. C. Toxicology and pharmacology}, doi = {10.1016/j.cbpc.2009.10.009}, volume = {151}, number = {2}, issn = {1532-0456}, title = {Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss)}, keyword = {ABC efflux transporters, xenobiotics, rainbow trout, mRNA expression, tissue distribution} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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