engleski

Immunoreactivity and frequency of gastrin cells - a comparative study

In the present study the immunoreactivities of gastrin (G-) cells of the human and rat antral stomach against specific high titer antisera of low concentrations were investigated by means of the unlabeled antibody enzyme (PAP) technique. Modifications of single steps in the immunocytochemical procedure sequence, i. e. substitution of primary antiserum with several peptide antisera, tests with antiimmunoglobulins and non-immune sera and elevation of salt content in PBS were performed. These experiments have shown that the polyvalent immunore immunoreactivities described by some authors in G-cells of rats are not paralleled in human G-cells. There are no signs of non-specific binding of immunoglobulins in human G-cells. Quantitative analysis of human fetal and neonatal antrum showed a significant increase of G-cells during intogenesis.

gastrin cells; human; rat; stomach; immunohistochemistry

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