engleski

Glycoconjugates and regulatory peptides in bovine abomasum during development

Six lectins with different major sugar-binding affinities wereinvestigated by indirect immunoperoxidase methods in paraffinsections of bovine abomasum during fetal and postnatal period. Inaddition gastrin and somatostatin were examined by PAP method inendocrine cells of same specimens. Ulex europeaus I agglutinin(UEA I) which recognizes L-fucose residue and Dolichos biflorusagglutinin (DBA) which recognizes Nac-D-acetilgalactosamineproved to be the best markers of stomach glands during theprocess of differentiation. In early stages of development (10 to14 wk of age) UEA I labels uniformely cell membranes ofepithelium clusters and later on cytoplasmic compartments inseparate regions of developing glands. With the progress ofdifferentiation at 20 to 22 wk of age, DBA strongly labelsprimitive parietal cells at the bottom of crypts. Postnatally andin adults DBA selectively labels intra- and intercellularcanaliculi of parietal cells depending on the secretory state.Stereological analysis of gastrin producing cells, which firstappeared as singles cells at 16th wk of gestation, showed asignificant increase of frequency (Vv=0.13 +- 0.001) and volumeof nuclei at 22th wk of age. This coincided with the beginning ofparietal cell proliferation and general growth of glands. Thefrequency of G-cells in adult mucosa reached Vv=0.28+-0.001. Themultistep process of development of bovine stomach mucosa isapparently accompanied by structural changes in glycoconjugatesand presumably by trophic action of gastrin.

Glycoconjugates; regulatory peptides; bovine abomasum; differentiation; immunoperoxidase method

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