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Olive and Corn-oil Enriched Diets and Mineral Content in the Brain during Liver Regeneration in Mice

Zinc, calcium, magnesium and iron are essential oligoelements. They play important role in various biological processes, including lipid metabolism. We have examined the influence of olive oil and corn oil enriched diets on zinc, calcium, magnesium and iron concentrations in brain in the early period of liver regeneration. The common procedures for mineral analysis of biological tissue is inductively coupled plasma-atomic emission spectromerty (ICP-AES). This technique requires total digestion of samples by using strong reactants. Sample preparation is the crucial first step in the analysis of this kind of samples. Minerals were determined by ICP-AES, after microwave brain tissue digestion by HNO3 and H2O2. Mineral content was estimated in the samples, and the results are presented as a dynamics of Zn2+, Ca2+, Mg2+, and Fe2+ found in the brain, in comparison with the changes found in the regenerating liver in mice fed with standard food and with vegetable oil enriched diet.

minerals; brain; partial hepatectomy; diet

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