engleski

Crystallography in bioanalysis

For characterization of a compound, either a small molecule or a macromolecule, information about structure and properties is needed. If the compound can be crystallized then X-ray diffraction analysis gives a lot of information about the molecular and crystal structure. Crstallization of proteins is difficult and can be a bottleneck for such analysis. Our newest results in protein crystallography will be briefly discussed: (1) enzymatic activity of tyrosine phenol-lyase (TPL) could be deduced by detailed structural analysis of native and mutated TPL forms in complexes with substrate analogues ; (2) 3-hydroxyanthranilate 3, 4-dioxygenase, enzyme that catalyzes the conversion of 3-hydroxyanthranilate to quinolinic acid, has been extracted and purified from bovine kidney, crystallized and its structure determined at 2.5 &Aring ; resolution ; (3) coordination of iron in bovine insulin changed upon addition of bromide or iodide ions into crystallization solutions.

crystallography; bioanalysis

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