engleski

Proteomic characterization of plasma-derived clotting factor VIII-von Willebrand factor concentrates

Proteomic methods were used to identify the levels of impurities in three comercial plasma-derived clotting factor VIII-von Willebrand factor (FVIII/VWF)concentrates. In all three concentrates, significant amounts of other plasma proteins were found. In Octanate and Haemoctin, two concentrates developed in the 1990s, the major impurities identified wereinter-alpha inhibitor proteins, fibrinogen and fibronectin. These two concentrates were also found to contain additional components such as clotting factor II (prothrombin) that are known activators of FVIII. In Wilate, a recently developed FVIII/VWF concentrate, the amount of these impurities was significantly reduced. Batch-to-batch variations and differeces between three investigated products were detected using iTRAQ, an isotope labeling technique for comparative MS, demonstrating the potential value of this technique for quality control analysis. The importance of through proteomic investigations of therapeutic FVIII/VWF preparations from human plasma is also discused.

clotting factor VIII; proteomic analysis; prothrombin; von Willebrand factor

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