engleski

Pentadecapeptide BPC 157 and Raman spectroscopy as a method for monitoring healing process in bone, intestinal anastomosis and stomach in rat.

We focused on Raman spectroscopy as a method for monitoring healing process i) bone healing, ii) intestinal anastomosis healing and iii) stomach healing. To accelerate and improve healing we used pentadecapeptide BPC 157, either ip application (10ug, 10 ng/kg, once daily) (i, ii) or topical application of one drop at the site of injury (2mg, 2ug, 2 ng, 2pg/ml) (iii) . i. Raman spectra were recorded at the edge of the circular bone defect of a rat skull, 3, 7, 14 and 21 days after the defect was induced. Some changes of characteristic Raman bands appeared in spectra recorded from bone of BPC 157 rats already after 3 and 7 days while in controls effect was observes after 14 days. ii. Raman spectra were recorded at the site of the intestinal anastomosis 1, 2, 3, 7 and 14 days after the ileoileal anatomosis was created. Differences of spectra were demonstrated using principal component analysis to maximize the spectral variances. Separations of the spectra with different healing period on PC scores scatterplot indicated some changes of Raman spectra recorded from intestine of BPC 157 rats. iii. The 3x3mm samples were excided from the rats stomach, and either kindly brushed or leaved intact, and one drop of distilled water with or without BPC 157 was inserted to the specimen through the holder. Five Raman spectra were recorded in intervals of 10 minutes. Principal component analysis was applied on all recorded spectra and separation according to concentration and time was noticed. Separations of the spectra with different post-application period and different BPC 157 concentrations on PC scores scatterplot indicated definitive changes of Raman spectra recorded from both damaged and non damaged stomach of BPC 157 rats in either of tested post-application intervals.

pentadecapeptide BPC 157 ; Raman spectroscopy

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