hrvatski

Protein expression of early genes of variant and referent HPV 16

The genome of the Human papillomavirus (HPV) 16, causing 60% cervical cancer and other human malignancies has been extensively studied. In our previous study, we identified a novel HPV 16 variant, which contain 63-nucleotides duplication in the E1 region (Sabol et al. J Med Virol 2008 ; 80:2134-40). This variant seems to be more associated to low grade cervical lesions and thus probably have a lower oncogenicity. In addition, sequence analysis of the E6 and the E7 regions of each 63-nucleotides E1 variant samples were also related to HPV 16 E6-G350 variant. This might clarify the differences of E6-G350 variant oncogenicity observed in European populations. In silico translation of the E1 gene with the duplication has shown that the protein has extra 21 amino acids as the duplication is “ in frame” and does not introduce a premature stop codon. The affected region has currently no significant function ascribed to it. In order to further analyze the differences between the variant and referent HPV 16 proteins we cloned E1, E2, E5, E6 and E7 early viral genes into the pQE 30-UA bacterial expression vectors. The E6 and E7 protein were successfully cloned and expressed, while expression experiments for E1, E2, and E5 are still under way. This is the first step in our goal to identify the cause of reduced oncogenicity of this novel HPV variant.

humani papilomavirusi (HPV); rani geni

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