engleski

Caging exposure of European seabass (Dicentrarchus labrax) for in situ assessment of pollution-related genotoxicity

Genotoxic effects are often one of the earliest signs of pollution-related environmental disturbance. In this study Comet assay and micronucleus test were applied to assess DNA damage in erythrocytes of European seabass (Dicentrarchus labrax) exposed to environmental pollution in situ. Fish were collected at the fish farm in the Trogir Bay and cage exposed for a period of four weeks at two sites of different pollution intensity along the Adriatic coast, reference site Šolta (Nečujam Bay) and polluted site Vranjic (Kaštela Bay). Fish collected at the fish farm Trogir Bay were used as a second control group. Statistically significant increase in DNA damage measured by the Comet assay was observed at the site Vranjic comparing to both reference sites. Micronuclei induction showed similar gradient of DNA damage, but did not reach the statistical significance. Our results indicated the applicability of caging exposure of a marine fish D. labrax in environmental biomonitoring and confirm the usefulness of the Comet assay as a suitable tool for detection of pollution-related genotoxicity.

Comet assay; micronucleus test; fish; marine biomonitoring; ecogenotoxicology; Adriatic Sea

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