engleski

Seroprevalence of equine viral arteritis in Croatia - variable specificity of immunoenzyme assay in different horse population

In the summer 2008 first confirmed outbreak of EVA in Croatia occurred on State horse farm of Lippizaner, Djakovo and a source of an outbreak is still under investigation. Object of this study was to investigate if EVA was imported for the first time 2008 or is it a wide distributed but not recognized disease in Croatia.To clarify former EAV prevalence in different horse populations in Croatia we used 200 archive horse sera randomly selected from horse sera collected during 2005. Specific antibodies were determinate by ELISA and VN-test. At the same time we investigated the specificity of ELISA assay depending on different populations, sexual maturity and gender of tested animals. Results showed that EVA is present in sport and leisure horses in Croatia for at least 4 years, but until 2008 was unrecognized. Also this study showed that ELISA specificity varies widely regarding population, age and gender of tested animals.

equine viral arteritis; seroprevalence; ELISA; VN-test; specificity

nije evidentirano