Toxic effects of metabolites of indoor moulds (CROSBI ID 554403)
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Podaci o odgovornosti
Pieckova, E ; Kollarikova, Z ; Kovačikova, Z ; Hurbankova, M ; Černa, S ; Liškova, A ; Tatrai, E ; Šegvić Klarić, Maja ; Bloom, E
engleski
Toxic effects of metabolites of indoor moulds
There are many reports of allergies, chronic intoxication or even lung hemosiderosis of workers and occupants in buildings contaminated with indoor moulds. Mechanisms of the potential pathological effects of fungal toxic compounds on airways of healthy humans have not been sufficiently clariffied yet. In vitro and in vivo toxicity of the most frequent indoor mycromycetes- under Slovak dwellings conditions (Aspergillus veriscolor, A. ustus, Penicillium expansum, P. Chrysogenum) and top risky Stachybotrys chartarum-from the public health point of view-with extrolites profile characterised by TLC and LC/MS/MS-was studied by microbiological methods (employing Bacillus subtilis), ba a bioassay on chick tracheal cultures and by analyses of histological and biochemical alterations of rat organs or cell cultures and cytotoxic, infalmmatory and hematological parameters in bronchoalveolar lavage fluid or blood. Pure solvent (2% dimethylsulfoxide-negative control) and tandards of mycotoxins, all carcinogenic, potentially produced by the fungus tested (sterigmatocystin, patulin, ochratoxin A, trichotecenes) were used-20 ug/mL (in vitro studies) and 4 ug/mL (in vivo, intratracheal instilation), exposure for 3 days. All fungal metabolites tested showed certain toxic effects (bacterial growth inhibition, caesing tracheal epithelial cells beating, statistically significant changes in hematological parameters and lung injury-cytotoxic and inflammatory-detectable by bronchoalveolar lavage) that were concetration and cell origin of the toxicant (exo- or endocellular metabolite) dependent. Generall, exometabolites were able to damage natural cell and organ functions of airways in vitro/in vivo much stronger.
A. veriscolor; Stachybotrys; sterigmatocystin; patulin; ochratoxin A
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Podaci o prilogu
107-107.
2008.
objavljeno
Podaci o matičnoj publikaciji
6th Pan African Environmental Mutagen Society Conference (PAEMS 2008)
Podaci o skupu
6th Pan African Environmental Mutagen Society Conference (PAEMS 2008)
poster
02.11.2008-05.11.2008
Cape Town, Južnoafrička Republika