engleski

In vitro antibiotic sensitivity of Pseudomonas aeruginosa isolates from different pathological conditions of animal

To determine in vitro sensitivity to selected antimicrobial agents, during seven years, strains of Pseudomonas (P.) aeruginosa were collected from clinical specimens. From 1993 through 2000 from 5344 examined clinical specimens total of 155 strains belonging to this species were isolated. The majority of strains (123 strains) originated from ear discharge of dogs with otitis externa, and the other strains were isolated from pathologically changed organs of dead animals, milk samples, urine, faeces and wounds. The strains were tested for susceptibility by disk-diffusion method using Mueller-Hinton agar against seven agents of known antipseudomonas activity, i.e. imipenem, pipemidic acid, carbenicillin, ceftazidime, cefoperazone, amikacin, and gentamicin. Imipenem was the most active of tested compounds, with 151 strains ( 97.4%) sensitive, one strain (0.6%) partial sensitive and three strains (1.9%) were resistant. Against cephalosporin cefoperazon (third generation) 140 strains (90.3%) showed sensitivity, eight were partial sensitive (5.2%) and seven (4.5%) were resistant. Aminoglycoside amikacin was active against 134 isolates (86.5%), nine of them were partial sensitive (5.8%), and 12 (7.7%) were resistant. Ceftazidime was active against 132 strains (85.2%), eight strains (5.2%) were partial sensitive and 15 were resistant (9.7%). To aminoglycoside gentamicin 129 strains (83.2%) were sensitive, nine (5.8%) were partial sensitive and 17 (10.6%) were resistant. Against carbenicillin 118 strains (76.1%) were sensitive, nine (5.8%) were partial sensitive and 28 strains (18.1%) were resistant. Significant difference in antipseudomonas activity to all antimicrobial agents was observed in pipemidic acid. Out of 155 tested strains, 62 were resistant (40.0%), 11 were partial sensitive (7.1%) and 82 strains (52.9%) were sensitive. Susceptibility tests showed that the majority of strains were sensitive to specific antipseudomonas agents, except the less active pipemidic acid.

Pseudomonas aeruginosa; antimicrobial susceptibility

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