engleski

Potential risks for the vertical transmission of Bovine Viral Diarrhoea (BVD) virus

The current breeds of beef cattle are selected for their high productivity and capacity for adaptation to farm breeding. In raising beef cattle, any disease transmission to the first filial generation can be prevented by implementing effective prevention and treatment strategies. The paper shows the results obtained in the study of beef cattle samples of the same herd conducted to determine the potential risk and occurrence of bovine viral diarrhoea (BVD) virus in filial calves. We tested 21 randomly selected bovine samples (17 bovine blood samples and 4 bull semen samples) from the BVD infected area. Previous studies by comparative analysis of 11 microsatellite loci have shown that the selected animals are interbred. The technique employed includes RNA extraction from bovine blood and bull semen samples using the QIAamp kit. The polymerase chain reaction (PCR) products are then analysed by gel electrophoresis to see whether there are any sequences indicating the presence of BVD virus in any of the study samples. Since the tested samples of bovine blood and bull semen of the same herd were determined negative for the presence of bovine viral diarrhoea (BVD) virus the assumption of potential infection transmission couldn't be confirmed nor refuted. The aim of this paper is to assess the risk of BVD virus transmission from parent to the next 4filial generation. In case there is a potential risk of transmission, the animals should be tested immediately after birth to determine whether they are infected by BDV virus. Since the assumption of potential infection transmission could be neitherconfirmed nor refuted in this study, the need for further research involving a larger number of blood samples, particularly bull semen samples, is underlined.

BVD; PCR; transmission

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