engleski

Animal model for age and sex related genotoxicity of diethylstilbestrol

Environmental xenoestrogens pose a significant health risk for all living organisms. There is growing evidence on different susceptibility to xenoestrogens in developing and adult organism, but little is known about their genotoxicity in pre-pubertal mammals. In this study we developed an animal model to show gender and age specific genotoxcity of a synthetic oestrogen diethylstilbestrol (DES). Genotoxicity of DES was tested on the reticulocytes of pre-pubertal and adult mice using the in vivo micronucleus (MN) assay. Diethylstilbestrol had been administered intraperitonealy (0.05 µ ; g/kg, 0.5 µ ; g/kg and 5 µ ; g/kg) for three days. Animals were sampled 48 h, 72 h, 96 h, and 2 weeks after exposure. After the dose of 0.05 µ ; g/kg of DES pre-pubertal mice showed a significant increase in MN frequency (P<0.001) while in adults it remained at reference values (5, 3 MN/1000 versus 1, 0 MN/1000 reticulocytes). At doses of 0.5 µ ; g/kg and 5 µ ; g/kg, MN frequency significantly increased in both age groups. In pre-pubertal male animals, the MN frequency remained above the reference values even two weeks after exposure. Our animal model for pre-pubertal genotoxicity assessment using in the vivo MN assay proved to be sensitive enough to distinguish age and sex differences in genome damage caused by DES. Diethylstilbestrol showed to be more genotoxic in prepuberal animals especially in males.

oestrogen ; puberty ; in vivo micronucleus assay ; gender ; genotoxicity

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